Chemistry
School of Mathematics & Physical Sciences

Prof Nicole Pamme

Diplom Chemiker (Marburg, Germany), PhD (Imperial College London),
DIC, FRSC, FHEA

Nicole Pamme

Professor in Analytical Chemistry

Chemistry

  • Profile
  • Teaching
  • Research
  • Key publications
  • Research Group

Profile

Nicole Pamme graduated with the title of Diplom Chemiker (equivalent to an MChem) from the University of Marburg, Germany, in 1999. For her PhD she went to Imperial College London to join the group of Prof. Andreas Manz, where she worked on single particle analysis in microfluidic chips until 2004. This was followed by a stay in Tsukuba, Japan, as an independent research fellow in the International Centre for Young Scientists (ICYS) at the National Institute for Materials Science NIMS. She has been a Lecturer in Analytical Chemistry at the University of Hull since 2005 and was promoted to Senior Lecturer in 2011, Reader in 2013 and Professor in 2014.

Departmental responsibilities

  • Director of Research (since 2014)
  • Deputy Head of Department (2014-2015)
  • Departmental REF lead (2011-2015)
  • Departmental Research Committee (since 2011)
  • Organiser of Departmental Seminar Series (2008-2012)
     

University responsibilities

  • Member of University Research Committee (2008-2010)

Teaching

Teaching interests

Analytical Chemistry

Research

Research theme: Chemical Process Technology
Research groups: Analytical Science, Lab on a Chip and Micro-fluidics

The current research themes in my group evolve around the study of microfluidic lab-on-a-chip devices for chemical and bioanalysis, chemical processing and medical diagnostics.

  1. Exploitation of Magnetic Attraction Forces »
    • Magnetic particles are commonly used in bioanalysis but conventional tube-based procedures are cumbersome. In our research group, we have developed continuous flow approaches for particle sorting and on-particle processing. These include continuous flow separation of magnetic microparticles and magnetically labelled cells based on their size and magnetic loading. More recently, we have presented platforms for continuous flow processing on particle surfaces allowing for entire analysis procedures to be conducted within one minute in an automated fashion. Processes studied so far include immunoassays and DNA hybridisation assays. And layer-by-layer deposition.

      Continuous flow separation (free-flow magnetophoresis)  image 1

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      Continuous flow processing on particle surfaces

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      Selected references

      C. Phurimsak, M.D. Tarn, S.A. Peyman, J. Greenman, N Pamme, On-chip determination of C-reactive protein using magnetic particles in continuous flow, Analytical Chemistry, 2014, 86, 10552-10559Chayakom CRP.

      M. Vojtisek, A. Iles, N. Pamme, Rapid, multi-step on-chip DNA hybridisation in continuous flow on magnetic particles, Biosensors and Bioelectronics, 2010, 25, 2172-2176.

      S.A. Peyman, A. Iles, N. Pamme, Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow, Lab on a Chip, 2009, 9, 3110-3117.

      S.A. Peyman, A. Iles,N. Pamme, Rapid on-chip multi-step (bio)chemical reactions in continuous flow – manoeuvring particles through co-laminar flow streams. Chemical Communications, 2008, 10, 1220-1222. 

      N. Pamme, A. Manz, On-Chip Free-Flow Magnetophoresis: Continuous flow separation of magnetic particles and agglomerates. Analytical Chemistry, 2004, 76, 7250-7256.

       

  1. Exploitation of Magnetic Repulsion Forces »
    • Polymer particle and biological cells are usually diamagnetic and thus weakly repelled from magnetic fields. This repulsion force can be enhanced by suspending particles or cells in a paramagnetic medium such as a gadolinium or manganese salt. In our research we have applied this principle for the trapping, focussing and deflection of polymer particles and cells and performed bioassays, pre-concentration and separation processes.

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      Selected references

      M.D. Tarn, L.T. Elders, S.A. Peyman, N. Pamme, Diamagnetic repulsion of particles for multilaminar flow assays, RSC Advances, 2015, 5, 103776-103781

      M.D. Tarn, S.A. Peyman, N. Pamme, Simultaneous trapping of magnetic and diamagnetic particle plugs for separations and bioassays, RSC Advances, 2013, 3, 7209–7214.

      M. Vojtisek, M.D. Tarn, N. Hirota, N. Pamme, Microfluidic devices in superconducting magnets: on-chip free-flow diamagnetophoresis of polymer particles and bubbles, Microfluidics and Nanofluidics, 2012, 13, 625-635.

      A.I. Rodríguez-Villarreal, M.D. Tarn, L.A. Madden, J.B. Lutz, J. Greenman, J. Samitier, N. Pamme, Flow focussing of particles and cells based on their intrinsic properties using a simple diamagnetic repulsion setup, Lab on a Chip, 2011, 11, 1240-1248.

      S.A. Peyman, E-Y. Kwan, O. Margarson, A. Iles, N. Pamme, Diamagnetic repulsion – a versatile tool for on-chip label-free particle handling, Journal of Chromatography A, 2009,  1216, 9055-9062.

  1. Magnetic Nanoparticles »
    • We are working with magnetic particles as labels for biological cells. We study nanoparticle uptake and therefore endocytotic capacity of macrophages and HeLa cells via our continuous flow magnetophoresis separation chips. We have labelled yeast cells with magnetic nanoparticles for on-chip layer deposition of polymeric materials using our multi-laminar flow devices towards formation of cyborg cells. Genetically modified GFP reporter yeast were also labelled with magnetic nanoparticles and trapped on a concentration gradient chip for toxicity studies based on GFP expression. Furthermore, we are working with highly concentrated magnetic nanoparticle suspensions, co-called ferrofluids, to generate pL vessels on chip that can be manipulated inside microfluidic systems by an external magnet.

      Studying Endocytosis through uptake of magnetic nanoparticles

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      On-chip layer deposition on magnetic yeast cells towards cyborg cells

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      GFP producing magnetic yeast cells to study toxicity in a concentration gradient on-chip

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      Ferrofluid droplets as pL reagent vessels generated and manoeuvred on-chip

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      Selected references

      D. Robert, N. Pamme, H. Conjeaud, F. Gazeau, A. Iles, C. Wilhelm, Cell sorting by endocytotic capacity in a microfluidic magnetophoresis device, Lab on a Chip, 2011, 11, 1902-1910.
      N. Pamme, C. Wilhelm, Continuous sorting of magnetic cells via on-chip free-flow magnetophoresis. Lab on a Chip, 2006, 6, 947-980.

      M.D. Tarn, R.F. Fakhrullin, V.N. Paunov, N. Pamme, Microfluidic device for the rapid coating of magnetic cells with polyelectrolytes, Materials Letters, 2013, 95, 182-185.

      J. García-Alonso, R.F. Fakhrullin, V.N. Paunov, Z. Shen, J.D. Hardege, N. Pamme, S.J. Haswell, G.M. Greenway, Micro-screening Toxicity System based on Living Magnetic Yeast and Gradient Chips, Analytical and Bioanalytical Chemistry, 2011, 400, 1009-1013.

      E. AlHetlani, O.J. Hatt, M. Vojtisek, M.D. Tarn, A. Iles, N. Pamme, Sorting and manipulation of magnetic droplets in continuous flow, American Institute of Physics Conference Proceedings, 2010, 1311, 167-175

  1. Photochemistry »
    • High surface-to-volume ratios and penetration distances make lab-on-a-chip devices favourable for photochemistry applications. We have utilised photochemistry for the controlled generation of reactive oxygen species ROS) by immobilisation of photosensitisers such as porphyrins on the channel walls and studied the effect on chemical oxidation reactions and biological cells. We have also developed a flow cell onto which cyanobacteria were immobilised and maintained and achieved efficient hydrogen generation under illumination. (c) Furthermore we have performed photopolymerisation in droplet microfluidics to generation amphiphilic hydrogels as drug delivery vesciles.

      Immobilisation of porphyrin photosensiter for controlled generation of ROS

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      Biohydrogen generation from fabric-immobilised Cyanobacteria

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      Photopolymerisation of droplets to from amphiphilic microgels for drug delivery

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      Selected references

      E.K Lumley, C.E. Dyer, N. Pamme, R.W. Boyle, Comparison of photo-oxidation reactions in batch and a new photosensitiser-immobilised microfluidic device, Organic Letters, 2012, 14, 5724-5727.

      A.A.K. Das, M.M.N. Esfahani, O.D. Velev, N. Pamme, V.N. Paunov, Artificial leaf device for hydrogen generation from immobilised C. reinhardtii microalgae, Journal of Materials Chemistry A, 2015, 3, 20698-20707.

      B. Lu, M.D. Tarn, N. Pamme, T.K. Georgiou, Microfluidically fabricated pH-responsive anionic amphiphilic microgels for drug release, Journal of Materials Chemistry B, 2016, doi: 10.1039/C5TB02378E.

      B. Lu, M.D. Tarn, N. Pamme, T.K. Georgiou, Tailoring pH-responsive acrylic acid microgels with hydrophobic crosslinks for drug release, Journal of Materials Chemistry B, 2015, 3, 4524 - 4529.

  1. Pre-concentration method »
    • We are developing microfluidic approaches to the processes and synthesis of radiopharmaceutics as utilised in PET and SPECT imaging. The motivation is to enable dose-on-demand synthesis and minimise infrastructure and lab-safety requirements due to the smaller radioactive volumes processes. We have employed particle beds and monoliths for pre-concentration. In the processing of radiopharmaceuticals for PET imagings, fluoride generated from the cyclotron needs to be pr-conctratioed prior to synthesis. We are also working with monolitis for the pre-concentraiton and purification of radioactive gallium from the gallium generator prior to synthesis.

      Particle beads for fluoride pre-concentration and radiotracer purification

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      Silica monoliths for solid-phase radiotracer synthesis

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      Selected references

      V. Arima, G. Pascali, O. Lade, H.R. Kretschmer, V. Hammond, P. Watts, F. De Leonardis, M.D. Tarn, N. Pamme, P. Dittrich, B. Cvetkovic, N. Vasovic, R. Duane, A. Jaksic, A. Zacheo, A. Zizzari, L. Marra, E. Perrone, P.A. Salvadori, R. Rinaldi, Radiochemistry on chip: towards dose-on-demand synthesis of PET radiopharmaceuticals, Lab on a Chip, 2013,13, 2328–2336.

      M.D. Tarn, G. Pascali, F. De Leonardis, P.A. Salvadori, P. Watts, N. Pamme, Purification of 2-deoxy-2-[18F]fluoro-D-glucose radiotracer by on-chip solid-phase extraction, Journal of Chromatrography A, 2013, 1280, 117-121.

      F. De Leonardis, G. Pascali, P.A. Salvadori, P. Watts,N. Pamme, On-chip pre-concentration and complexation of [18F]fluoride ions via a regenerable anion exchange particles for radiochemical synthesis of Positron Emission Tomography tracers, Journal of Chromatography A, 2011, 29, 1218, 4714-4719.

      P. He, S.J. Haswell, N. Pamme, S. Archibald , Quantitative isolation of 68Ga from 68Ge/68Ga generator eluates using strong cation-exchange silica-monolith, 2016, under review.

  1. Quality Control For (Radio)Pharmaceuticals »
    • Pharmaceuticals must undergo stringent quality control before release to the patient. In the context of radiopharmaceutical for PET imaging, this includes physical, chemical, radiochemical and biological purity. Current quality control systems required an entire dose. In the context of dose-on-demand synthesis of single doses, QC tests must be minimised and automated.

      We are developing an integrated QC platform, featuring fibre optical absorbance spectrometers for pH, kryptant and endotoxin analysis, Raman spectroscopy for residual solvents, separation systems in conjunction with electrochemical and novel methods for radiation detection at the small scale.

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      Selected references

      M.D. Tarn, D. Maneuski, R. Alexander, N.J. Brown, V. O’Shea, SL. Pimlott, N. Pamme, S.J. Archibald, Positron detection in silica monoliths for miniaturised quality control of PET radiotracers, Chemical Communications, 2016, doi: 10.1039/C5CC10518H.

      M.P. Taggart, M.D. Tarn, M.M.N. Esfahani, D.M. Schofield, N.J. Brown, S.J. Archibald, T. Deakin, N. Pamme, Lee F. Thompson, Development of radiodetection systems towards miniaturised quality control of PET and SPECT radiopharmaceuticals, Lab on a Chip, 2016, doi 10.1039/C6LC00099A

  1. Food Pathogens »
    • Worked with bioMerieux on isolation of pathogens from food and blood samples employed acsoutohporesis and magnetophoresis.

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      Selected References:
      B. Ngamsom, M.M.N. Esfahani, C. Phurimsak, M.J. Lopez-Martinez, J-C. Raymond, P. Broyer, P. Patel, N. Pamme, Multiplex Sorting of Foodborne Pathogens by on-chip Free-Flow Magnetophoresis, Analytica Chimica Acta, 2016, 918, 69-76.

      B. Ngamson, M.J. Lopez-Martinez, J-C. Raymond, P. Broyer, P. Patel,N. Pamme, On-chip acoustophoretic isolation of microflora including S. typhimurium from raw chicken, beef and blood samples, Journal of Microbiological Methods, 2016, 123, 79-86.

  1. IFAST for Pathogen Analysis »
    • We have applied the technique of immiscible filtration assisted by surface tension (IFAST) to magnetically capture and isolate pathogen DNA from urine and stool sample for analysis of STDs and infection.

      IFAST as applied for pathogen analysis in urine and stool samples

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      Selected references

      O. Mosley, L. Melling, M.D. Tarn, C. Kemp, M.M.N. Esfahani, N. Pamme, Kirsty J. Shaw, Sample introduction interface for on-chip nucleic acid-based detection of Helicobacter pylori from stool samples, Lab on a Chip, 2016, under revision.

      C. Kemp, J. Wojciechowska, M.M.N. Esfahani, G. Benazzi, K.J. Shaw, S.J. Haswell, N. Pamme. On-chip processing and DNA extraction from large volume urine samples for the detection of Herpes simplex virus 2. Proceedings: T. Fujii et al. (eds), Chemical and Biological Microsystems Society, 2012, 911-913.

  1. Integrated DNA Analysis »
    • We have worked with diagnostics company Randox to develop a lab-on-a-chip platform for multiplex analysis if 10 sexually transmitted infections. Processing steps included pathogen cell lysis followed by capture of DNA on silanol surface magnetic particles, on-chip amplifaicoatn followed by a hydrodisaiton array with chemiluminesence readout.

      Multiplex analysis of 10 STDs

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  1. Tissue on-chip/Organs-on-chip »
    • In collaboration with biologists and medical researchers, the lab-on-a-chip team at the University of Hull has undertaken extensive work to demonstrate the maintenance of patient derived tissue biopsies on-chip. We are now adapting this concept to precision cut tissue slices for longitudinal studies of therapy based on tissue imaging and effluent analysis.

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Key publications

Recent and representative publications

Positron detection in silica monoliths for miniaturised quality control of PET radiotracers,
Mark D. Tarn, Dzmitry Maneuski, Richard Alexander, Nathaniel J. Brown, Val O’Shea, Sally L. Pimlott, Nicole Pamme, Stephen J. Archibald, Chemical Communications, 2016, doi: 10.1039/C5CC10518H.

Development of radiodetection systems towards miniaturised quality control of PET and SPECT radiopharmaceuticals,
Matthew P. Taggart, Mark D. Tarn, Mohammad M. N. Esfahani, Daniel M. Schofield, Nathaniel J. Brown, Stephen J. Archibald, Tom Deakin, Nicole Pamme, Lee F. Thompson, Lab on a Chip, 2016, doi 10.1039/C6LC00099A

Multiplex Sorting of Foodborne Pathogens by on-chip Free-Flow Magnetophoresis,
Bongkot Ngamsom, Mohammad Mehdi Nasr Esfahani, Chayakom Phurimsak, Maria J. Lopez-Martinez, Jean-Claude Raymond, Patrick Broyer, Pradip Patel, Nicole Pamme, Analytica Chimica Acta, 2016, 918, 69-76. doi: 10.1016/j.aca.2016.03.014

Lab-on-a-chip workshop activities for secondary school students,
Mohammad M. N. Esfahani, Mark D. Tarn, Tahmina A. Choudhury, Laura C. Hewitt, Ashley J. Mayo, Theodore A. Rubin, Mathew R. Waller, Martin G. Christensen, Amy Dawson, Nicole Pamme, Biomicrofluidics, 2016, 10, 011301. doi: 10.1063/1.4940884

Microfluidically fabricated pH-responsive anionic amphiphilic microgels for drug release,
Bingyuan Lu, Mark D. Tarn, Nicole Pamme, Theoni K. Georgiou, Journal of Materials Chemistry B, 2016, doi: 10.1039/C5TB02378E

Artificial leaf device for hydrogen generation from immobilised C. reinhardtii microalgae,
Anupam A. K. Das, Mohammad M. N. Esfahani, Orlin D. Velev, Nicole Pamme, Vesselin N. Paunov, Journal of Materials Chemistry A, 2015, 3, 20698-20707. doi: 10.1039/C5TA07112G

On-chip determination of C-reactive protein using magnetic particles in continuous flow,
Chayakom Phurimsak, Mark D. Tarn, Sally A. Peyman, John Greenman, Nicole Pamme, Analytical Chemistry, 2014, 86, 10552-10559. doi: 10.1021/ac5023265

Phaseguide assisted liquid lamination for magnetic bead-based assays,
Chayakom Phurimsak, Ender Yildirim, Sebastiaan J. Trietsch, Thomas Hankemeier, Mark D. Tarn, Nicole Pamme, Paul Vulto, Lab on a Chip, 2014, 14, 2334-2343. doi: 10.1039/C4LC00139G

Full Publication List

Positron detection in silica monoliths for miniaturised quality control of PET radiotracers, Mark D. Tarn, Dzmitry Maneuski, Richard Alexander, Nathaniel J. Brown, Val O’Shea, Sally L. Pimlott, Nicole Pamme, Stephen J. Archibald, Chemical Communications, 2016, doi: 10.1039/C5CC10518H.

Development of radiodetection systems towards miniaturised quality control of PET and SPECT radiopharmaceuticals,
Matthew P. Taggart, Mark D. Tarn, Mohammad M. N. Esfahani, Daniel M. Schofield, Nathaniel J. Brown, Stephen J. Archibald, Tom Deakin, Nicole Pamme, Lee F. Thompson, Lab on a Chip, 2016, doi 10.1039/C6LC00099A

Lab-on-a-chip workshop activities for secondary school students,
Mohammad M. N. Esfahani, Mark D. Tarn, Tahmina A. Choudhury, Laura C. Hewitt, Ashley J. Mayo, Theodore A. Rubin, Mathew R. Waller, Martin G. Christensen, Amy Dawson, Nicole Pamme, Biomicrofluidics, 2016, doi: 10.1063/1.4940884

Multiplex Sorting of Foodborne Pathogens by on-chip Free-Flow Magnetophoresis,
Bongkot Ngamsom, Mohammad Mehdi Nasr Esfahani, Chayakom Phurimsak, Maria J. Lopez-Martinez, Jean-Claude Raymond, Patrick Broyer, Pradip Patel, Nicole Pamme, Analytica Chimica Acta, 2016, 918, 69-76. doi: 10.1016/j.aca.2016.03.014

On-chip acoustophoretic isolation of microflora including S. typhimurium from raw chicken, beef and blood samples,
Bongkot Ngamson, Maria J. Lopez-Martinez, Jean-Claude Raymond, Patrick Broyer, Pradip Patel, Nicole Pamme, Journal of Microbiological Methods, 2016, 123, 79-86. doi: 10.1016/j.mimet.2016.01.016

Microfluidically fabricated pH-responsive anionic amphiphilic microgels for drug release,
Bingyuan Lu, Mark D. Tarn, Nicole Pamme, Theoni K. Georgiou, Journal of Materials Chemistry B, 2016, doi: 10.1039/C5TB02378E

Diamagnetic repulsion of particles for multilaminar flow assays,
Mark D. Tarn, Luke T. Elders, Sally A. Peyman, Nicole Pamme, RSC Advances, 2015, 5, 103776-103781. doi: 10.1039/C5RA21867E

Artificial leaf device for hydrogen generation from immobilised C. reinhardtii microalgae,
Anupam A. K. Das, Mohammad M. N. Esfahani, Orlin D. Velev, Nicole Pamme, Vesselin N. Paunov, Journal of Materials Chemistry A, 2015, 3, 20698-20707. doi: 10.1039/C5TA07112G

Tailoring pH-responsive acrylic acid microgels with hydrophobic crosslinks for drug release, Bingyuan Lu, Mark D. Tarn, Nicole Pamme, Theoni K. Georgiou, Journal of Materials Chemistry B, 2015, 3, 4524 - 4529. doi: 10.1039/C5TB00222B

On-chip determination of C-reactive protein using magnetic particles in continuous flow,
Chayakom Phurimsak, Mark D. Tarn, Sally A. Peyman, John Greenman, Nicole Pamme, Analytical Chemistry, 2014, 86, 10552-10559. doi: 10.1021/ac5023265

Advances in processes for PET radiotracer synthesis: separation of [18F]fluoride from enriched [18O]water,
Ping He, Stephen J. Haswell, Nicole Pamme, Stephen J. Archibald, Applied Radiation and Isotopes, 2014, 91, 64-70. doi: 10.1016/j.apradiso.2014.04.021

Phaseguide assisted liquid lamination for magnetic bead-based assays,
Chayakom Phurimsak, Ender Yildirim, Sebastiaan J. Trietsch, Thomas Hankemeier, Mark D. Tarn, Nicole Pamme, Paul Vulto, Lab on a Chip, 2014, 14, 2334-2343. doi: 10.1039/C4LC00139G

On-chip processing of particles and cells via multilaminar flow streams,
Mark D. Tarn, Maria Lopez-Martinez, Nicole Pamme, Analytical and Bioanalytical Chemistry, 2014, 406, 139-161. doi: 10.1007/s00216-013-7363-6

Simultaneous trapping of magnetic and diamagnetic particle plugs for separations and bioassays,
Mark D. Tarn, Sally A. Peyman, Nicole Pamme, RSC Advances, 2013, 3, 7209–7214. doi: 10.1039/C3RA40237A

Radiochemistry on chip: towards dose-on-demand synthesis of PET radiopharmaceuticals,
Valentina Arima, Giancarlo Pascali, Oliver Lade, Hans Richard Kretschmer, Victoria Hammond, Paul Watts, Francesco De Leonardis, Mark D. Tarn, Nicole Pamme, Petra Dittrich, Benjamin Cvetkovic, Nikola Vasovic, Russell Duane, Aleksandar Jaksic, Antonella Zacheo, Alessandra Zizzari, Lucia Marra, Elisabetta Perrone, Piero A. Salvadori, Rosaria Rinaldi, Lab on a Chip, 2013,13, 2328–2336. doi: 10.1039/C3LC00055A

Purification of 2-deoxy-2-[18F]fluoro-D-glucose radiotracer by on-chip solid-phase extraction,
Mark D. Tarn, Giancarlo Pascali, Francesco De Leonardis, Piero A. Salvadori, Paul Watts, Nicole Pamme, Journal of Chromatrography A, 2013, 1280, 117-121.
doi: 10.1016/j.chroma.2013.01.032

Microfluidic device for the rapid coating of magnetic cells with polyelectrolytes,
Mark D. Tarn, Rawil. F. Fakhrullin, Vesselin N. Paunov, Nicole Pamme, Materials Letters, 2013, 95, 182-185. doi: 10.1016/j.matlet.2012.12.084

Comparison of photo-oxidation reactions in batch and a new photosensitiser-immobilised microfluidic device,
Emily K Lumley, Charlotte E. Dyer, Nicole Pamme, Ross W. Boyle, Organic Letters, 2012, 14, 5724-5727. doi: 10.1021/ol3023424

Microfluidic devices in superconducting magnets: on-chip free-flow diamagnetophoresis of polymer particles and bubbles,
Martin Vojtisek, Mark Tarn, Noriyuki Hirota, Nicole Pamme, Microfluidics and Nanofluidics, 2012, 13, 625-635. doi: 10.1007/s10404-012-0979-6

On-chip bioanalysis with magnetic particles,
Nicole Pamme, Current Opinion in Chemical Biology, 2012, 16, 243-246. doi: 10.1016/j.cbpa.2012.05.181

Integrated DNA extraction and amplification using electrokinetic pumping in a microfluidic device,
Joseph Parton, Christopher Birch, Cordula Kemp, Stephen J. Haswell, Nicole Pamme, Kirsty J. Shaw, Analytical Methods, 2012, 4, 96-100. doi: 10.1039/C1AY05552F

Microfluidic platforms for performing surface-based clinical assays,
Mark D. Tarn, Nicole Pamme, Expert Review of Molecular Diagnostics, 2011, 11, 711-720. doi: 10.1586/erm.11.59  

Microfluidic modules for the preconcentration of [18F]-fluoride for PET radiotracer synthesis, and its subsequent removal during product purification,
Francesco De Leonardis, Mark D. Tarn, Giancarlo Pascali, Piero A. Salvadori, Nicole Pamme, Journal of Labelled Compounds and Radiopharmaceuticals, 2011, 54, S 46. doi: 10.1002/jlcr.1925

On-chip pre-concentration and complexation of [18F]fluoride ions via a regenerable anion exchange particles for radiochemical synthesis of Positron Emission Tomography tracers,
Francesco De Leonardis, Giancarlo Pascali, Piero A. Salvadori, Paul Watts, Nicole Pamme, Journal of Chromatography A, 2011, 29, 1218, 4714-4719. doi: 10.1016/j.chroma.2011.05.062

Cell sorting by endocytotic capacity in a microfluidic magnetophoresis device,
Damien Robert, Nicole Pamme, Hélène Conjeaud, Florence Gazeau, Alexander Iles, Claire Wilhelm, Lab on a Chip, 2011, 11, 1902-1910. doi: 10.1039/c0lc00656d

Micro-screening Toxicity System based on Living Magnetic Yeast and Gradient Chips,
Javier García-Alonso, Rawil F. Fakhrullin, Vesselin N. Paunov, Zheng Shen, Joerg D. Hardege, Nicole Pamme, Stephen J. Haswell, Gillian M. Greenway, Analytical and Bioanalytical Chemistry, 2011, 400, 1009-1013. doi: 10.1007/s00216-010-4241-3

Flow focussing of particles and cells based on their intrinsic properties using a simple diamagnetic repulsion setup,
Angeles Ivón Rodríguez-Villarreal, Mark D. Tarn, Leigh A. Madden, Julia B. Lutz, John Greenman, Josep Samitier, Nicole Pamme, Lab on a Chip, 2011, 11, 1240-1248. doi: 10.1039/c0lc00464b

On-chip regeneration of anion exchange resins for [18F-] pre-concentration in PET radiochemical synthesis,
Francesco De Leonardis, Giancarlo Pascali, Piero A. Salvadori, Nicole Pamme, The Quarterly Journal of Nuclear Medicine and Molecular Imaging, 2010, 54 (2, S1), 22-25.

Sorting and manipulation of magnetic droplets in continuous flow,
Entesar AlHetlani, Oliver J. Hatt, Martin Vojtisek, Mark D. Tarn, Alexander Iles, Nicole Pamme, American Institute of Physics Conference Proceedings, 2010, 1311, 167-175. doi:10.1063/1.3530008

Rapid, multi-step on-chip DNA hybridisation in continuous flow on magnetic particles,
Martin Vojtisek, Alexander Iles, Nicole Pamme, Biosensors and Bioelectronics, 2010, 25, 2172-2176. doi: 10.1016/j.bios.2010.01.034

Diamagnetic repulsion – a versatile tool for on-chip label-free particle handling,
Sally A. Peyman, Er-Yee Kwan, Oliver Margarson, Alexander Iles, Nicole Pamme, Journal of Chromatography A, 2009,  1216, 9055-9062. doi: 10.1016/j.chroma.2009.06.039

Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow,
Sally A. Peyman, Alexander Iles, Nicole Pamme, Lab on a Chip, 2009, 9, 3110-3117. doi: 10.1039/b904724g

A microfluidic system for performing fast, sequential biochemical procedures on the surface of mobile magnetic particles in continuous flow,
Sally A. Peyman, Herika Patel, Naomi Belli, Alexander Iles, Nicole Pamme, Magnetohydrodynamics, 2009, 45, 361-370. doi: 10.1586/erm.11.59

The importance of particle type selection and temperature control for on-chip free-flow magneto-phoresis,
Mark D. Tarn, Sally A. Peyman, Damien Robert, Alexander Iles, Claire Wilhelm, Nicole Pamme, 2009, Journal of Magnetism and Magnetic Materials, 2009, 321, 4115-4122. doi: 0.1016/j.jmmm.2009.08.016

On-chip diamagnetic repulsion in continuous flow,
Mark D. Tarn, Noriyuki Hirota, Alexander Iles, Nicole Pamme, Science and Technology of Advanced Materials, 2009, 10, 014611.

Rapid on-chip multi-step (bio)chemical reactions in continuous flow – manoeuvring particles through co-laminar flow streams,
Sally A. Peyman, Alexander Iles, Nicole Pamme, Chemical Communications, 2008, 10, 1220-1222. doi: 10.1039/B716532C

Simultaneous Bioassays in a microfluidic channel on plugs of different magnetic particles,
Sandrine Bronzeau, Nicole Pamme, Analytica Chimica Acta, 2008, 609, 105-112. doi: 10.1016/j.aca.2007.11.035

Critical Review: Continuous flow separations in microfluidic devices,
Nicole Pamme, Lab on a Chip, 2007, 7, 1644-1659. doi: 10.1039/B712784G

Bonding of soda lime glass microchips at low temperature,
Alexander Iles, Akio Oki, Nicole Pamme, Microfluidics and Nanofluidics, 2007, 3, 119-122. doi: 10.1007/s10404-006-0101-z

Continuous sorting of magnetic cells via on-chip free-flow magnetophoresis,
Nicole Pamme, Claire Wilhelm, Lab on a Chip, 2006, 6, 947-980. doi: 10.1039/B604542A

On-Chip Free-Flow Magnetophoresis: Separation and detection of mixtures of magnetic particles in continuous flow,
Nicole Pamme, Jan C.T. Eijkel, Andreas Manz, Journal of Magnetism and Magnetic Materials, 2006, 307, 237-244. doi: 10.1016/j.jmmm.2006.04.008

Critical Review: Magnetism in microfluidic chips,
Nicole Pamme, Lab on a Chip, 2006, 6, 34-38. doi: 10.1039/B513005K

On-Chip Free-Flow Magnetophoresis: Continuous flow separation of magnetic particles and agglomerates,
Nicole Pamme, Andreas Manz, Analytical Chemistry, 2004, 76, 7250-7256. doi: 10.1021/ac049183o

Counting and sizing of particles and particle agglomerates in a microfluidic device using laser light scattering: application to a particle-enhanced immunoassay,
Nicole Pamme, Ryuji Koyama, Andreas Manz, Lab on a Chip, 2003, 3, 187-192. doi: 10.1039/B300876B

Analysis of polynitrophenols and hexyl by liquid chromatography – mass spectrometry using atmospheric pressure ionisation methods and a volatile ion-pairing reagent,
Nicole Pamme, Klaus Steinbach, Wolfgang J. Ensinger, Torsten C. Schmidt, Journal of Chromatography A, 2001, 943, 47-54. doi: 10.1016/S0021-9673(01)01430-3

Textbooks, Book Chapters

Bioanalytical Chemistry (2nd edition) Andreas Manz, Petra Dittrich, Nicole Pamme, Dimitri Iossifidis, Imperial College Press, London, 2015, ISBN: 978-1783266722 (textbook for undergraduate students, 200 pp).

Microfluidics, Mark Tarn, Nicole Pamme,
in Elsevier Online Reference Database in Chemistry, Molecular Sciences and Chemical Engineering, http://dx.doi.org/10.1016/B978-0-12-409547-2.05351-8 , 2013.

Magnetic nanoparticles in Lab-on-a-chip devices, Nicole Pamme,
in Magnetic Nanoparticles: From Fabrication to Clinical Applications, Nguyen TK Thanh (ed.), CRC Press, Taylor and Francis Group, 2012, ISBN: 9781439869321.

Sputtering for Thin Film Deposition, Alexander Iles, Nicole Pamme
in: Encyclopedia of Micro- and Nanofluidics
editor Dongqing Li, Springer, Heidelberg, 2008.

Bioanalytical Chemistry, Andreas Manz, Nicole Pamme, Dimitri Iossifidis,
Imperial College Press, London, 2004, ISBN: 978-1860943713.

Research Group

PostDoctoral Research Assistants

  • Dr Bongkot Ngamsom (Newton Fund, British Council)
    Nov. 2015 – Oct. 2016
    Water Quality Monitoring in Resource Poor Environments
  • Dr Mark Tarn (Daisy Charity, HEIF)
    since Sep. 2012
    Lab-on-a-chip based analysis of radiopharmaceuticals
  • Dr Ping He (Daisy Charity, HEIF)
    since July 2011
    Lab-on-a-Chip based synthesis of PET radiopharmaceuticals
  • Dr Nasr Esfahani (Daisy Charity, HEIF)
    since Aug. 2014
    Manufacturing of integrated synthesis and analysis platforms for PET radiopharmaceuticals

PhD students

  • Chayakom Phurimsak (main supervisor)
    July 2010 – June 2013
    Clinical diagnostics in continuous flow
  • Mohammad Chowdhury (co-supervisor)
    Oct. 2010 - Sep. 2013
    Engineering design for miniature biochemical analysis systems
  • Zahra Ghobaei-Namhil (co-supervisor)
    Oct. 2011 - Sep, 2014
    Nanogap bio-Sensors for DNA analysis
  • Anupam Das (co-supervisor)
    Oct. 2011 - Sep. 2014
    Bio-hydrogen production by using artificial symbiotic biofilms of cyanobacteria
  • Kapiraj Chandrabalan (co-supervisor)
    Oct. 2011 - Sep. 2014
    A microfluidics approach to studying the role of oxidative stress in cellular transformation and disease induction
  • Ali-AlOrabi (main supervisor
    June 2012 - May 2015
    On-chip assembly of drug and reagent delivery vesicles
  • Bingyuan Lu (co-supervisor)
    Nov. 2012 - Oct. 2015
    Smart microgels fabricated on lab-on-a-chip devices

Former PostDoctoral Research Assistants

  • Dr Bongkot Ngamsom (EU-FP7)
    Apr 2014 – Mar 2015
    Network for Innovation and Learning on Microreactor Technology
  • Dr Bongkot Ngamsom (industrial sponsor)
    Sep 2013 – Mar 2014
    Microbial purification on-chip from industrial matrices
  • Dr Martin Vojtisek (TSB)
    Feb 2013 – Jan 2014
    Development of a rapid multiplex Lab on a Chip system for detection of 10 STI pathogens using Biochip Array Technology
  • Dr Emily Lumley (EU-FP7)
    Feb 2013 – Aug 2013
    Network for Innovation and Learning on Microreactor Technology
  • Dr Mark Tarn (EU-FP7)
    Sep 2012 – Aug 2013
    Microscale radiopharmaceutical synthesis
  • Dr Maria Lopez-Martinez (industrial sponsor)
    Feb 2012 – Aug 2013
    Microbial purification on-chip from industrial matrices
  • Dr Cordula Kemp (TSB)
    Feb 2011 – Jan 2013
    Development of a rapid multiplex Lab on a Chip system for detection of 10 STI pathogens using Biochip Array Technology

Former PhD students

  • Rachel Smith (co-supervisor)
    Oct. 2009 - Sep. 2012
    On-chip synthesis of anti-cancer drugs
  • Emily K. Lumley (co-supervisor)
    Oct. 2008 - Sep. 2011
    Development of a microfluidic device for the in-situ production of singlet oxygen for chemical and biological appliations
  • Francesco de Leonardis (main supervisor)
    Oct. 2008 - Sep. 2011
    Microfluidic modules for pre-concentration of [18F]fluoride in positron emission tomography (PET) radiotracer synthesis
  • Martin Vojtisek (main supervisor)
    July 2008 - June 2011
    Magnetic manipulation of particles for microfluidic continuous flow processes
  • Mark D. Tarn (main supervisor)
    Oct. 2007 - Sep. 2010
    Continuous flow processes on single magnetic and diamagnetic particles in microfluidic devices
  • Entesar AlHetlani (co-supervisor)
    Sep. 2007 - Aug. 2012
    Generation and manipulation of magnetic droplets in microfluidic devices
  • Sally A. Peyman (main supervisor)
    May 2006 - Oct. 2009
    Bioanalysis on single magnetic particles
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